Dendrimer supramolecular assembly for gene delivery / Karthikeyan Pasupathy

Public ISBD Titre : Dendrimer supramolecular assembly for gene delivery Type de document : texte imprimé Auteurs : Karthikeyan Pasupathy Editeur : Ann Arbor : ProQuest LLC Année de publication : 2008 Importance : ii, 58 p. ISBN/ISSN/EAN : 978-0-549-69649-0 Langues : Anglais (eng) Tags : DENDRIMERS  MOLECULAR BIOLOGY  BIOPHYSICS Index. décimale : EQ -Equipe- Résumé : "Dendrimers have found many applications in the fields of polymer science, biophysics, nanomedicine and the petroleum industry. Poly(amidoamine) (PAMAM) was studied as a model dendrimer and squalane as a model hydrocarbon. The interaction between PAMAM and squalane is pH dependent. Specifically, at low or neutral pH the squalane is found on the periphery of the PAMAM while at high pH the hydrocarbon is entrapped inside the PAMAM molecules. Single-molecule fluorescence revealed that the interaction between PAMAM and squalane is reversible. At a pH value of 8, the time constants for the approaching, binding and dissociation of single PAMAM to squalane are 0.5s, 7.5s and 0.5s respectively. Spectrophotometric measurements revealed these interactions increase for dendrimers of lower generations. PAMAM was found to be an effective vector for plant gene delivery. By optimizing the pH of the plant growth medium and the molar ratio of dendrimer to plasmid DNA, the expression of Green Fluorescent Protein (GFP) delivered by PAMAM is possible in turfgrass cells. In principle, this method could be extended to other plant cells by optimizing the dendrimer delivery system as necessary. Such novel scheme overcomes the pitfalls of conventional gene delivery means involving intensive labor, cost and host range limitations." Cote : LCC/M Num_Inv : 3201 Dendrimer supramolecular assembly for gene delivery [texte imprimé] / Karthikeyan Pasupathy . - Ann Arbor : ProQuest LLC, 2008 . - ii, 58 p. ISBN : 978-0-549-69649-0 Langues : Anglais (eng) Tags : DENDRIMERS  MOLECULAR BIOLOGY  BIOPHYSICS Index. décimale : EQ -Equipe- Résumé : "Dendrimers have found many applications in the fields of polymer science, biophysics, nanomedicine and the petroleum industry. Poly(amidoamine) (PAMAM) was studied as a model dendrimer and squalane as a model hydrocarbon. The interaction between PAMAM and squalane is pH dependent. Specifically, at low or neutral pH the squalane is found on the periphery of the PAMAM while at high pH the hydrocarbon is entrapped inside the PAMAM molecules. Single-molecule fluorescence revealed that the interaction between PAMAM and squalane is reversible. At a pH value of 8, the time constants for the approaching, binding and dissociation of single PAMAM to squalane are 0.5s, 7.5s and 0.5s respectively. Spectrophotometric measurements revealed these interactions increase for dendrimers of lower generations. PAMAM was found to be an effective vector for plant gene delivery. By optimizing the pH of the plant growth medium and the molar ratio of dendrimer to plasmid DNA, the expression of Green Fluorescent Protein (GFP) delivered by PAMAM is possible in turfgrass cells. In principle, this method could be extended to other plant cells by optimizing the dendrimer delivery system as necessary. Such novel scheme overcomes the pitfalls of conventional gene delivery means involving intensive labor, cost and host range limitations." Cote : LCC/M Num_Inv : 3201

Exemplaires(1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
3201	LCC/M	Texte imprimé	Equipe	Livre	Equipe - Demande préalable Disponible

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